Last updated on 2019-09-30 by Xiang-Jun Lu <xiangjun@x3dna.org>.
The block schematics were created with DSSR and
rendered using PyMOL.
- PDB-id
- 6CCG
- Class
- transcription
- Method
- X-ray (1.9 Å)
- Summary
- Crystal structure mbd3 mbd domain in complex with methylated cpg DNA
List of 4 5mC-amino acid contacts:
-
C.5CM6: stacking-with-A.ARG22 is-WC-paired is-in-duplex [+]:GcG/cGC
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D.5CM6: stacking-with-A.ARG44 is-WC-paired is-in-duplex [-]:cGC/GcG
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E.5CM6: stacking-with-B.ARG22 is-WC-paired is-in-duplex [+]:GcG/cGC
-
F.5CM6: stacking-with-B.ARG44 is-WC-paired is-in-duplex [-]:cGC/GcG
direct SNAP output · DNAproDB 2.0
- Reference
- Liu, K., Lei, M., Wu, Z., Gan, B., Cheng, H., Li, Y., Min, J.: (2019) "Structural analyses reveal that MBD3 is a methylated CG binder." Febs J., 286, 3240-3254.
- Abstract
- The MBD3, a methyl-CpG-binding domain (MBD)-containing protein, is a core subunit of the Mi-2/NuRD complex. Recent reports show that MBD3 recognizes both methylated CG (mCG)- and hydroxymethylated CG (hmCG)-containing DNA, with a preference for hmCG. However, whether the MBD3-MBD indeed has methyl-CG-binding ability is controversial. In this study, we provided the structural basis to support the ability of MBD3-MBD to bind mCG-containing DNA. We found that the MBD3-MBD bound to mCG-containing DNA through two conserved arginine fingers, and preferentially bound to mCG over hmCG, similar to other methyl-CpG-binding MBD proteins. Compared to its closest homolog MBD2, the tyrosine-to-phenylalanine substitution at Phe34 of MBD3 is responsible for a weaker mCG DNA binding ability. Based on the complex structure of MBD3-MBD with a nonpalindromic AmCGC DNA, we suggest that all the mCG-binding MBD domains can recognize mCG-containing DNA without orientation selectivity, consistent with our observations that the sequences outside the mCG dinucleotide do not affect mCG DNA binding significantly. DNA cytosine methylation is evolutionarily conserved in most metazoans, and most invertebrates have only one MBD gene, MBD2/3. We also looked into the mCG DNA binding ability of some invertebrates MBD2/3 and found that the conserved arginine fingers and a conserved structural fold are required for methylated DNA binding by MBD2/3-MBDs in invertebrates. Hence, our results demonstrate that mCG-binding arginine fingers embedded into a conserved structural fold are essential structural features for MBD2/3s binding to methylated DNA among metazoans.
- The 5-methylcytosine group (PDB ligand '5CM') is shown in space-filling model,
with the methyl-carbon atom in black.
- Watson-Crick base pairs are represented as long rectangular blocks with the
minor-groove edge in black. Color code: A-T red, C-G yellow, G-C green, T-A blue.
- Protein is shown as cartoon in purple. DNA backbones are shown ribbon, colored code
by chain identifier.
- The block schematics were created with 3DNA-DSSR,
and images were rendered using PyMOL.
- Download the PyMOL session file corresponding to the top-left
image in the following panel.
- The contacts include paired nucleotides (mostly a G in G-C pairing), and
amino-acids within a 4.5-A distance cutoff to the base atoms of 5mC.
- The structure is oriented in the 'standard' base reference frame of 5mC, allowing for easy comparison
and direct superimposition between entries.
- The black sphere (•) denotes the 5-methyl carbon atom in 5mC.
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No. 1 C.5CM6: download PDB file
for the 5mC entry
stacking-with-A.ARG22 is-WC-paired is-in-duplex [+]:GcG/cGC
|
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No. 2 D.5CM6: download PDB file
for the 5mC entry
stacking-with-A.ARG44 is-WC-paired is-in-duplex [-]:cGC/GcG
|
 |
No. 3 E.5CM6: download PDB file
for the 5mC entry
stacking-with-B.ARG22 is-WC-paired is-in-duplex [+]:GcG/cGC
|
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No. 4 F.5CM6: download PDB file
for the 5mC entry
stacking-with-B.ARG44 is-WC-paired is-in-duplex [-]:cGC/GcG
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